Joint CeNS Colloquium with SFB1032

Title: Synthetic Cells: De Novo Engineering with DNA Nanotechnology
Location: Faculty of Physics, Kleiner Physikhörsaal N020 and the talk will be streamed online via Zoom
Host: Prof. Erwin Frey, Prof. Dieter Braun

see also CeNS-colloquiums-website

Summary:

Can we construct a cell from non-living matter? In search for answers, bottom-up synthetic biology has successfully encapsulated functional sets of biomolecules inside lipid vesicles, yet a “living” synthetic cell remains unattained. Instead of relying exclusively on biological building blocks, the integration of new tools can be a shortcut towards the assembly of active and eventually fully functional synthetic cells. This is especially apparent when considering recent advances in DNA nanotechnology. DNA nanotechnology allowed us to engineer various functional parts for synthetic cells, which, meanwhile have found diverse applications as biophysical probes in cell biology. Recently, we engineered functional DNA-based mimics of a cytoskeleton. These cytoskeletons are capable of stimuli-responsive reversible assembly, cargo transport and can deform giant unilamellar lipid vesicles (GUVs) from within. We further demonstrate the division of GUVs based on phase separation or spontaneous curvture increase and osmosis rather than the biological building blocks of a cell’s division machinery. We derive a parameter-free analytical model which makes quantitative predictions that we verify experimentally. The osmolarity increase can be triggered by enzymatic reactions or by light-triggered release of caged compounds. Ultimately, by coupling GUV division to their informational content and their function, we aim for a prototype of a synthetic cell capable of evolution.