SFB 1032: Nanoagents for Spatiotemporal Control of Molecular and Cellular Reactions

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Nanoimaging of DNA nanoagents interacting with cells

Investigating the localization, abundance, and mutual interactions of components on the plasma membrane such as receptors and their ligands, is critical for various biological processes such as cell signalling or carcinogenesis. However, their interaction mechanisms are poorly understood and manipulating them in a prescribed fashion on the molecular scale has been difficult to achieve.
In this project, we will apply tools from DNA Nanotechnology such as DNA-PAINT, a recently devel-oped DNA-based imaging modality, and lattice light-sheet microscopy to perform programmable, multiplexed, live cell imaging of cell surface receptor dynamics, study the interaction with their lig-ands as well as trigger clustering of multiple receptors using DNA origami nanoagents displaying specific ligands in prescribed quantities and geometries.